NEET Biology - New - Biotechnology: Principles & Processes

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Question - 1

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

  • A The larger the fragment size, the farther it moves
  • B The smaller the fragment size, the farther it moves
  • C Positively charged fragments move to farther end
  • D Negatively charged fragments do not move

Question - 2

A gene whose expression helps to identify transformed cell is known as :

  • A Selectable marker
  • B Vector
  • C Plasmid
  • D Structural gene

Question - 3

Which of the following restriction enzymes produces blunt ends?

  • A Sal I
  • B Eeo RV
  • C Xho I
  • D Hind III

Question - 4

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:

  • A Eeo RI
  • B Taq polymerase
  • C Polymerase III
  • D Ligase

Question - 5

Which of the following is not a feature of the plasmid?

  • A Single stranded
  • B Independent replication
  • C Circular structure
  • D Small, circular double-stranded

Question - 6

In genetic engineering, the antibiotics are used.

  • A As selectable markers
  • B To select healthy vectors
  • C As sequences from where replication starts
  • D To keep the culture free ofinfection

Question - 7

The given figure is the diagrammatic representation of E.coli vector pBR 322.

Which one of the given options correctly identifies its certain component(s)?

  • A Ori - original restriction enzyme
  • B Rop - Reduced osmotic pressure
  • C Hind III, EcoR I - selectable markers
  • D AmpR, tetR - antibiotic resistance genes

Question - 8

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands?
5'_________GAATTC__________3'
3'_________CTTAAG__________5'
What is so special shown in it?

  • A Replication completed
  • B Deletion mutation
  • C Start codon at the 5' end
  • D Palindromic sequence of base pairs

Question - 9

The correct order of steps in Polymerase Chain Reaction (PCR) is :

  • A Denaturation, Extension, Annealing
  • B Annealing, Extension, Denaturation
  • C Extension, Denaturation, Annealing
  • D Denaturation, Annealing, Extension

Question - 10

Which of the following is not a component of downstream processing?

  • A Separation
  • B Purification
  • C Preservation
  • D Expression
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